75-1400 ng/dL) and estradiol 11
To measure total testosterone and estradiol in serum, CDC uses highly precise and accurate laboratory methods that employ High Performance Liquid Chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS)
The CDC Hormone Standardization (HoSt) Program is improving clinical assays, as evidenced by a 50% decline in mean absolute bias between mass spectrometry assays
The CDC HoSt program also includes serum estradiol measurement and regularly evaluates the performance of the different assays available
Sensitive simultaneous quantitation of testosterone and estradiol in serum by LC–MS/MS without derivatization and comparison with the CDC HoSt program
CDC HoSt; Estradiol; Liquid chromatography; Mass spectrometry; Method correlation; NIST SRM 971; Testosterone
Estradiol levels decline greatly with age
• CDC HoSt Program certifies the performance of participants within th e concentration range of 1
Within-day (N=10) and between-day (N=20) CVs at concentrations spanning the AMR were less than 7% for
Reliable measurement of total testosterone and estradiol is critical for their use as biomarkers of hormone-related disorders in patient care and translational research
Jan 9, 2022 #33 Microdosing Enanthate Slightly being defined as past the upper quartile but not double the norm
This data is also presented in “Sensitive Simultaneous Quantitation of Testosterone and Estradiol in Serum by LC-MS/MS without Derivatization and Comparison with the CDC HoSt Program” (Schofield et al
6% to 0
J Chromatogr B Biomed Appl The assay was calibrated with readily available commercial calibrators and compared with international reference standards
the cdc host program to improve testosterone and estradiol measurements The CDC HoSt Program helps in establishing traceability of measurements performed in patient care to a common reference material by assigning target values to individual donor sera using its reference methods
Sensitive measurements of estradiol (E 2) are needed for determination of menopausal status, estrogen deficiency, A comparison with 40 samples obtained from the CDC HoSt program showed that E 2 had a regression slope of 1
CDC offers up to 120 different samples for more detailed method verification and characterization for Total 25-hydroxyvitamin D, Total Testosterone and Estradiol
, 2017)
D
It is certified by CDC Hormone Standardization Program (HoSt) and is traceable to certified reference materials obtained from the National Metrology Institute of Japan, NMIJ CRM 6004-a for estradiol) and the Australian National Measurement Institute, ANMI M914, Australian for testosterone) (Zhou, et al, 2017)